FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l07n01 lbd38_-chx_4.34100000033f99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l07n01 lbd38_-chx_4.34100000033f99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11268446
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG501250.4448261987500317No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG460150.4083526690370615No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA326960.290155359487901No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC237430.2107034102129078TruSeq Adapter, Index 4 (100% over 51bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC226850.20131436047171014No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT179590.15937423847085924No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT172940.15347280361462443No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC156260.13867040761432411No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG154570.13717064447040878No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG152650.1354667715495109No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC143220.12709827069322602No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT138150.12259898126148006No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG135480.12022953298085645No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA132250.11736312176497098No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT131590.1167774154484123No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC124790.11074286552023233No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG121130.10749485776477075No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG118950.1055602520525013No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT115310.10232999297329907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32850.035.54560511
CACACGT34250.034.22402212
GTATGCC33000.034.1598745
CGTATGC33650.033.50002344
ACGTCTG35200.033.4287915
CACGTCT36750.031.95764514
ACTGACC36200.031.75895732
ACACGTC37200.031.57049813
TCGTATG36500.031.00742343
CTCGTAT36900.030.67129542
AGCACAC38600.030.48293110
CGTCTGA42600.027.9918616
AGTCACT41400.027.98654428
CACTGAC41100.027.97248531
GAGCACA43100.027.3524599
CAGTCAC43050.026.75734327
GTCACTG45250.026.00311929
TCTCGTA43450.025.99483741
CTGAACT45900.025.78261619
TCGGAAG47400.025.0742153
Produced by FastQC (version 0.11.2)