FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l07n01 lbd38_-chx_3.34100000033f62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l07n01 lbd38_-chx_3.34100000033f62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13622471
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG674790.4953506599500193No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG546340.4010579284771464No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA370110.2716907967724798No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC299390.21977657357464736No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT255170.18731550245179454No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG240580.1766052575924001No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT225640.16563808430937382No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG204940.15044260325457842No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC203210.14917264276062692TruSeq Adapter, Index 3 (100% over 51bp)
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC193160.1417951265963422No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT190470.13982044814042915No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT183920.13501221621246248No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC182980.13432217987470849No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA168310.1235532085184839No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT167890.12324489441012573No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC154360.11331277563373046No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG151960.11155098072882666No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA150380.11039113241643164No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG150280.11031772429539398No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA148980.10936341872190442No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG147560.10832102340316967No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA139610.10248507778067577No Hit
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG138110.10138395596511088No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT137460.10090680317836609No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATGT136480.10018740359219704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG31200.033.02782415
CGTATGC30400.032.7895844
GCACACG31900.031.87918911
GTATGCC31600.031.61561645
ACACGTC33400.030.5148513
CACTTAG32550.030.2742331
ACTTAGG33350.029.95343832
CACACGT34550.029.8247612
TCGTATG34450.029.26125343
TAGGCAT34450.028.47496235
CTCGTAT35450.028.1819442
CACGTCT36300.028.01556214
AGCACAC38150.026.83235710
TTAGGCA37450.026.2538134
TCACTTA38200.025.9142930
CGTCTGA40050.025.56119516
CATCTCG39900.025.03773939
TCTCGTA41450.024.1012941
AGTCACT42200.023.5108428
CAGTCAC43300.023.06952527
Produced by FastQC (version 0.11.2)