FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l07n01 lbd38_-chx_1.34100000033ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l07n01 lbd38_-chx_1.34100000033ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13673779
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG631340.4617158138946081No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG553540.4048185947717891No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA404490.2958143465679824No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC303890.2222428781392474No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT247470.1809814243743445No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG246400.18019890477972478No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG222590.16278601548262553No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT216740.15850775414755497No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT203900.149117519012118No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC190870.13958833179913177No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT189670.13871073973039932No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA175440.12830396044868064No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC174630.12771158580228625No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG169000.1235942163464833No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT167040.12216081596755365No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA155750.11390413725422943No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC150250.10988184027253914No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG147070.10755622129039821No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA146530.1071613048594686No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC142620.1043018173688488No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA142170.10397272034307414No Hit
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG140400.1026782720416938No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG137550.1005939908784543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT52850.022.3210741
CTGACTC60800.019.3996772
ACTCTAG61150.019.325385
GACTCTA64900.018.3128554
CTAGCAG71150.016.3161498
TGACTCT74900.016.0481973
CCGGGGT10450.015.2957531
CTCTAGC79800.014.9704376
TAGCAGA81000.014.3042499
GCGGGGC5900.014.1181741
CGCGGGG6450.012.9142971
CGGGCAT8950.012.8285021
GCGGGGA16450.012.727581
GGGGGAT14050.012.33795451
GCCGGGT10800.012.2986381
GCCGGGG9550.012.2582581
CTATCGA95450.012.21031517
TCTATCG96250.012.10882616
CGGGCGG9850.011.884911
GATCGGG87150.011.87581616
Produced by FastQC (version 0.11.2)