FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_9.341000000340d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_9.341000000340d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15758204
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG377780.239735441932342No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG356800.22642174197008746No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA254650.16159836488980597No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC159220.10103943317398355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20200.023.75451511
CGTATGC22500.021.12662944
GTATGCC23050.020.4279745
ACGTCTG26300.018.15867615
AGCACAC27700.017.56562810
GTCACGA27200.017.31054529
CTCGTAT27800.017.0988942
AGTCACG27800.017.01758828
TCGTATG29350.016.88344443
GCTGACT44100.016.8131471
CACACGT29100.016.797612
ACACGTC30250.015.86246813
CACGTCT31600.015.39737714
TCACGAT32800.014.69687430
GACTCTA57500.014.2393234
ACTCTAG58900.013.86278255
CGTCTGA35150.013.84191316
CTAGCAG60150.013.6436078
GGGGGAT15350.013.3032781
CTGACTC56200.013.1919182
Produced by FastQC (version 0.11.2)