FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_8.34100000034088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_8.34100000034088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10421009
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG249070.23900756634986112No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG215890.20716803910254758No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA175290.16820828002355626No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG112970.10840600943728194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12900.021.55228211
GTATGCC13400.020.91531645
CGTATGC14600.020.1176744
GCTGACT32050.019.6607631
AGCACAC16200.017.30040710
CTCGTAT17300.016.32990642
ACGTCTG17300.016.19991515
CTAGCAG43400.016.0668958
TCGTATG18250.015.97127943
ACTCTAG43600.015.6917955
CACACGT18050.015.52723412
GACTCTA45800.015.4278154
CTGACTC42250.015.0273942
CGGGCGA3100.014.9114731
GGGGGAT10500.014.8292541
CGCGGGG6550.014.1146781
CACGTCT20450.013.9239814
ACACGTC19650.013.92055413
GCGGGCT4200.013.9024281
GGGGGAC5400.013.516251
Produced by FastQC (version 0.11.2)