FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_7.3410000003402b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_7.3410000003402b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12920943
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG294000.22753757214159986No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG267130.20674187634757No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA212490.16445394117132162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13750.021.36233911
CGTATGC15600.017.82244144
GCTGACT39500.017.5487381
GTATGCC17700.015.45456145
CCGGGGT6700.014.8835731
ACGTCTG19750.014.87165215
AGCACAC19450.014.87123210
GACTCTA52400.014.7682634
ACTCTAG52450.014.6258875
CTCGTAT18750.014.469521542
CTAGCAG53500.014.4591298
CACACGT20350.014.21364612
CTGACTC49350.013.8970242
ACACGTC20850.013.65770913
CGGGGGT6450.013.5750341
CACGTCT22950.013.09178814
TCGTATG21750.012.9891743
CGGGCGA4900.012.4091511
CGCGGGG7450.012.0793511
GCCGGGT12100.012.0604651
Produced by FastQC (version 0.11.2)