FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_6.34100000033fb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_6.34100000033fb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13708184
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG327730.23907616063513593No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG288000.21009347408817974No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA233430.17028513769584652No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG142500.1039525001998806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15350.020.3041711
GTATGCC16000.019.47853545
CGTATGC16750.018.47250244
GCTGACT40350.017.784121
TCACGCC17200.017.33704830
CGCGGGG7400.016.7645681
GACTCTA54450.015.8186954
CTCGTAT20550.015.60219342
ACGTCTG19700.015.36453615
ACTCTAG55700.015.1818065
CTAGCAG54200.015.0171038
AGCACAC21300.014.94815610
TCGTATG21950.014.81135943
CACACGT21500.014.70481312
AGTCACG20750.014.695106528
GTCACGC20200.014.65124429
CACGCCA21650.014.08422331
ACGCCCC3550.013.7025242
TATCTCG23650.013.27238139
ACACGTC23300.013.18379113
Produced by FastQC (version 0.11.2)