FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_5.34100000033f7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_5.34100000033f7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13832066
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG302510.21870196397269936No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG271050.19595771159565029No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA223750.16176180767211493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT41000.018.6221391
GCACACG14050.018.19346211
GACTCTA53800.016.0110954
ACTCTAG55100.015.7147055
CGTATGC16550.015.5803544
CTAGCAG56600.015.1332498
GTATGCC17100.014.94810445
CTGACTC55800.013.9862672
CGGGCCT4700.013.4511441
GGGGGAT14650.013.4440821
ACGTCTG19400.013.40638415
CTCGTAT19700.012.74762242
TGACTCT70550.012.5594963
AGCACAC20450.012.38997310
CTCTAGC70850.012.3734926
ACACGTC21500.012.20204513
CCGGGGT7400.012.1577651
TAGCAGA70250.012.0012589
GGGGGAC7300.011.9912211
CTATCGA70700.011.98747717
Produced by FastQC (version 0.11.2)