FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_4.34100000033f49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_4.34100000033f49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13621141
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG255380.18748796448109598No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG223610.16416392723634532No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA184130.13517957122681573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17500.024.6003611
CGTATGC19850.020.21892444
GTATGCC20550.019.53020345
AGCACAC23700.018.44864810
ACTGACC22150.018.4223132
ACGTCTG24550.017.80858815
GCTGACT37750.017.6562981
ACACGTC25200.017.35051313
CTCGTAT24350.017.1269342
CACACGT25950.016.50343312
TCGTATG26150.016.03376243
CACGTCT28850.015.31054814
CGTCTGA28800.015.10279216
GACTCTA55150.014.3572134
CTAGCAG51400.014.1774648
CGCGGGG6450.013.95431
GGGGGAT14150.013.9249821
GAGCACA32800.013.8771559
ACTCTAG53850.013.8707355
CCGGGGT6450.013.5771571
Produced by FastQC (version 0.11.2)