FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_3.34100000033f05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_3.34100000033f05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15215936
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG334050.2195395669382416No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG283600.18638353894232992No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA238680.156861858514652No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG153590.10094022477486761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT43650.019.3095551
GCACACG13450.019.0056611
CGCGGGG9050.016.3722481
GACTCTA60250.016.3068544
ACTCTAG59750.016.1429795
CTAGCAG60200.015.6813918
CTGACTC58750.014.9651082
GTATGCC16750.014.859405545
GCCGGGG11150.013.5065341
CGTATGC17550.013.41545644
CCGGGGT9000.013.2245421
CTCTAGC75550.012.880636
AGCACAC19500.012.76401510
TGACTCT77000.012.7595843
TAGCAGA75250.012.6345079
CGGGCGC3707.2759576E-1212.473231
TAGGCAT19750.012.26135535
CACTTAG21150.012.19180131
CGGGCGA5600.012.1449881
ACTTAGG21250.012.13442932
Produced by FastQC (version 0.11.2)