FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_2.34100000033edd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_2.34100000033edd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12992296
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG284740.21916064720200343No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG256820.19767098902303334No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA212960.16391252169747364No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG136020.10469281180170156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13850.019.91247411
GCTGACT36900.019.6424661
CGTATGC16000.017.09686344
ACTCTAG52100.016.3601135
GTATGCC16850.015.96827145
CTAGCAG53600.015.6032098
GACTCTA56250.015.4721134
ACGTCTG19400.014.21526215
AGCACAC20300.014.02741210
CTGACTC52800.013.9102132
CACACGT21100.013.8143612
CGCGGGG7100.013.3601811
CTCTAGC65600.013.2610446
CTCGTAT21050.012.88872142
TAGCAGA66550.012.8365069
ACACGTC21850.012.51924813
CTATCGA67550.012.38050417
TGACTCT69850.012.3311963
TATCTCG22300.012.16588539
TCGTATG22900.012.04331943
Produced by FastQC (version 0.11.2)