FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_12.34100000034187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_12.34100000034187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15506165
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG361110.23288156678327618No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG321210.20714986587592743No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA249690.1610262756780932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT42500.019.2857741
GCACACG15900.018.61467711
CGCGGGG8500.016.5960671
GTATGCC17800.016.24929245
CGTATGC18400.016.20684844
CTAGCAG58000.015.65689858
ACTCTAG61600.015.5847095
GACTCTA63300.015.1661624
CACACGT21900.014.43622812
AGCACAC21250.014.350224510
GGGGGAT15950.014.1813761
CTGACTC59900.013.7228712
CCGGGGT7450.013.3851641
ACGTCTG22100.013.29031815
GCGGGCT8650.013.2153251
CTCTAGC76450.012.7282066
CTCGTAT22700.012.64296242
ACACGTC24350.012.24700713
TAGCAGA78000.012.1022539
TGACTCT80600.011.7160893
Produced by FastQC (version 0.11.2)