FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_11.3410000003416d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_11.3410000003416d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14027780
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG339100.24173461517075406No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG282970.20172115616298517No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA243130.1733203685829119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18850.024.38735611
CGTATGC21100.020.7240444
GTATGCC21100.020.51148445
ACGTCTG23100.019.51130115
AGCACAC23750.019.16702310
CGGCTAC23500.018.70196333
AGTCACG24600.018.04793728
CACACGT25600.017.6943112
CTCGTAT25050.017.3666642
ACGGCTA25900.017.0554832
GCTGACT37100.016.8718931
CCGGGGT7650.016.8086341
ACACGTC26850.016.70351813
CACGTCT28250.016.35171114
TCGTATG28700.015.78307843
GCTACAT27850.015.62002635
CACGGCT28450.015.60559731
GGGGCCT5400.015.2757691
GGCTACA29600.015.07510734
CGTCTGA30850.014.97339816
Produced by FastQC (version 0.11.2)