FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_10.34100000034100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_10.34100000034100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15649930
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG399800.2554644014382173No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG345380.2206910829633104No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA277490.17731069723634546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21000.023.59659411
CGTATGC22200.022.42203144
GTATGCC23500.021.18166445
GCTGACT42600.018.8421651
ACGTCTG26550.018.66271815
CACACGT28500.017.46563712
AGCACAC28750.017.39175210
CTCGTAT28750.017.31370542
TCGTATG28950.016.8842943
TAGCTTA29850.016.59955834
ACACGTC30000.016.5175113
TATCTCG30550.016.2195739
CACTAGC31000.015.4050731
CACGTCT32600.015.1999214
ACTCTAG59500.015.1925115
GACTCTA60800.015.1259194
ACTAGCT32900.014.99244932
CAGTCAC32250.014.9471627
GTCACTA32600.014.648993529
CGTCTGA34200.014.61941816
Produced by FastQC (version 0.11.2)