FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l06n01 lbd38_wchx_1.34100000033eb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l06n01 lbd38_wchx_1.34100000033eb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14701667
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG304910.2073982494638193No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG258790.176027657271791No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA223850.15226164488693697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT42650.018.6372181
GACTCTA56700.015.4673754
ACTCTAG56400.015.2314975
CTAGCAG57100.014.6864928
CTGACTC55450.014.4213762
CCGGGGT8000.012.7617931
GGGGGAT15050.012.2752261
TGACTCT75850.012.0354333
CTCTAGC73700.011.9559896
CGGGGGT8350.011.9357521
CGCGGGG8400.011.8647061
CGGGCGC4550.011.7534041
TAGCAGA72750.011.7428789
CTATCGA73500.011.3786917
GCCGGGT12100.011.2500661
TCTATCG74950.011.15855616
GGACGTC116000.010.82421437
CGGGCAT9250.010.7744351
CGGGGAG14650.010.6192721
CGGGCCG3751.7085767E-810.3714891
Produced by FastQC (version 0.11.2)