FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_9.34100000034095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_9.34100000034095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14151335
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21600.026.14739611
CGTATGC22150.025.39242444
GTATGCC23250.024.48135645
AGTCACG26150.022.02458228
ACGTCTG26800.021.49199915
CTCGTAT26500.021.3104142
TCGTATG27800.020.5554443
AGCACAC28650.020.18445210
ACACGTC28500.019.89592613
CACACGT29950.019.23320412
CGTCTGA33050.017.630516
GAGCACA34100.017.156459
TCTCGTA34150.016.60251241
AGAGCAC37350.016.264718
CAGTCAC36200.016.15861727
CACGTCT35500.016.03600114
GCGGGCT9200.014.43689251
CACGATC40700.014.37193631
TCGGAAG43850.013.8085393
TCACGAT41350.013.71075430
Produced by FastQC (version 0.11.2)