FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_8.34100000034045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_8.34100000034045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12912794
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA132150.10234036104037592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21000.027.42563244
GCACACG21600.027.18953511
GTATGCC21300.026.72248845
TCGTATG23200.025.11584343
CACACGT24200.024.82623912
ACACGTC24200.024.64027413
ACGTCTG24100.024.45947515
CTCGTAT25150.023.17028842
GTCACAC27700.020.8736229
AGCACAC30800.019.94467510
CGTCTGA30500.019.6946116
CACGTCT31550.018.970514
TCTCGTA30600.018.89649841
CAGTCAC35150.016.64149727
GAGCACA37500.016.561249
TCGGAAG39050.015.7357033
TCACACT38350.015.42859430
AGAGCAC39050.015.2114648
ATCTCGT38950.015.0188140
AGTCACA38900.014.86373328
Produced by FastQC (version 0.11.2)