FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_7.34100000033ff6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_7.34100000033ff6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11290608
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC150750.1335180532350428TruSeq Adapter, Index 7 (100% over 51bp)
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA121920.10798355588999282No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAAGGA114740.10162428808085447No Hit
GCCAAATGTTTGAAGGAATTGGTACCGAGTCTTAGGCGCCGGTGGAGTGGC113330.10037546250830778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24250.031.6358444
GCACACG25250.031.45798311
CACACGT26550.030.08717212
GTATGCC25750.029.96771445
ACGTCTG26850.029.57959215
ACACGTC26900.029.27745213
TCGTATG27550.028.00975243
CTCGTAT29250.026.07556242
AGCACAC32200.024.8777810
CACGTCT32300.024.2422614
CGTCTGA32900.024.0020316
CATCTCG33450.023.07054339
AGTCACC33900.023.0288528
TCTCGTA34500.022.36839541
GAGCACA37350.021.628259
CAGTCAC36650.021.4850627
AGAGCAC38900.020.8809438
TCGGAAG39300.020.2188973
TCTGAAC41800.019.3759518
CTGAACT43000.018.62594419
Produced by FastQC (version 0.11.2)