FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_6.34100000033fcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_6.34100000033fcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14522529
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG25100.027.07347111
CGTATGC24900.026.11156844
GTATGCC25600.025.4854645
TCACGCC26450.024.5812330
ACACGTC27600.024.29505513
ACGTCTG28950.023.70399915
GTCACGC27450.023.60378329
CACACGT29700.022.95603812
AGCACAC30700.022.57475710
AGTCACG29300.022.0366628
TCGTATG29600.021.88939543
CTCGTAT30300.021.38480442
CACGCCA31150.020.87234531
TATCTCG32600.020.49718739
CGTCTGA36150.019.04340416
CAGTCAC34850.018.78545427
GAGCACA37950.018.380649
AGAGCAC38050.018.2715638
CACGTCT37500.018.00061214
GCCAATA36050.017.91187334
Produced by FastQC (version 0.11.2)