FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_5.34100000033f8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_5.34100000033f8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13900987
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23850.028.68158511
CGTATGC24750.027.08785644
GTATGCC26500.025.29903645
ACGTCTG28900.024.21216615
CTCGTAT28250.023.57383542
ACACGTC30600.022.5753713
CACACGT30600.022.50183712
AGCACAC31000.022.35666310
TCGTATG30400.022.20144543
CGTCTGA35350.019.85624916
CACGTCT37800.018.92943414
CACAGTG35800.018.85398133
GAGCACA37200.018.6910429
ACACAGT36400.018.29595632
CACACAG37450.018.1420831
TCTCGTA37350.018.13146641
GTCACAC38450.017.61211629
AGAGCAC40750.017.4477068
CAGTCAC38950.017.21274827
TCGGAAG46000.015.4637353
Produced by FastQC (version 0.11.2)