FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_4.34100000033f56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_4.34100000033f56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12348541
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA150190.12162570460753218No Hit
GCCAAATGTTTGAAGGAATTGGTACCGAGTCTTAGGCGCCGGTGGAGTGGC147310.11929344527422307No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT139200.11272586777660615No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAAGGA138040.11178648554513444No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC130590.10575338414473417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17350.025.80770911
CGTATGC17700.025.16659544
ACGTCTG17950.024.69235215
GTATGCC18800.023.45474645
CACACGT19300.023.43336112
ACACGTC19350.023.14024513
CTCGTAT21150.020.9559742
TCGTATG21350.020.6533643
ACTGACC22650.019.56831432
AGCACAC23450.019.28630610
CGTCTGA25750.017.64852516
CACGTCT25450.017.59357514
CACTGAC25200.017.58726131
AGTCACT26750.016.40024628
TCTCGTA27550.016.25112341
GAGCACA29100.016.0829689
GCCGGGG10350.015.8804051
AGAGCAC31850.014.905368
CAGTCAC29700.014.84701627
TCGGAAG33400.014.0875893
Produced by FastQC (version 0.11.2)