FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_3.34100000033f12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_3.34100000033f12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13883002
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA185380.13353019757542353No Hit
GCCAAATGTTTGAAGGAATTGGTACCGAGTCTTAGGCGCCGGTGGAGTGGC184500.13289632890638495No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAAGGA183080.13187349537225448No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT157860.11370739556185326No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC140280.10104442828719609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21200.028.97416911
CGTATGC20900.028.63337944
ACGTCTG23400.026.7288515
GTATGCC22700.026.3628945
CACACGT24150.025.62121412
CTCGTAT24150.024.96786742
ACTTAGG23500.024.79716132
ACACGTC24900.024.75912913
CACTTAG24100.024.27132831
TCGTATG24700.024.22824343
TAGGCAT25500.023.02875135
TTAGGCA26700.021.99375334
AGCACAC29450.021.39227110
CGTCTGA30550.020.69342416
CATCTCG30600.020.29324339
TCACTTA30350.019.64375130
TCTCGTA31250.019.36716741
CACGTCT33000.018.88609514
AGTCACT34100.018.07748828
CAGTCAC34150.017.58986327
Produced by FastQC (version 0.11.2)