FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_2.34100000033eea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_2.34100000033eea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14321789
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17700.021.99273111
GTATGCC18350.020.71980545
CGTATGC18850.020.0508644
ACGTCTG20850.018.56022315
CACACGT22600.017.62264812
ACACGTC21800.017.54683313
TCGTATG22350.016.81018443
AGCACAC24200.016.55049510
CTCGTAT22800.016.47938242
TATCTCG23550.016.14574839
GAGCACA29000.014.1990549
CGTCTGA27800.014.00031616
GCGGGCT9600.013.836711
CACGTCT30150.013.20958614
AGAGCAC30200.013.1125698
CCGATGT31650.012.36909333
CGATGTA30600.012.35245334
CAGTCAC32650.011.85173227
AGTCACC32150.011.75614328
GCCGGGG12700.011.7001731
Produced by FastQC (version 0.11.2)