FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_12.34100000034144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_12.34100000034144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13888399
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC171380.12339795249258032TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC27650.030.43054245
GCACACG28050.030.40276111
CGTATGC27950.030.34539244
CACACGT30450.028.22817412
TCGTATG30250.028.1125143
ACGTCTG31300.027.67446915
ACACGTC31050.027.61023313
CTCGTAT31450.027.11304942
AGCACAC35250.024.51200310
TCTCGTA37300.023.04168941
CGTCTGA38650.022.4683416
CACGTCT39700.021.70744714
AGTCACC40400.021.27256828
CAGTCAC40800.020.40231527
GAGCACA42700.020.235329
AGAGCAC45050.019.0286168
TCGGAAG45800.018.9225753
CCAGTCA48200.017.83011826
ATCTCGT49600.017.32778440
CTGAACT52050.016.90009919
Produced by FastQC (version 0.11.2)