FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_11.3410000003412a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_11.3410000003412a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13842752
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC171530.12391322187958001TruSeq Adapter, Index 11 (100% over 51bp)
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA143900.10395331795296195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27450.030.9845211
CGTATGC27000.030.83023544
ACGTCTG29550.028.70404615
CACACGT29750.028.66471112
AGTCACG29400.028.39061728
GTATGCC29250.028.15101445
ACACGTC30100.028.03238713
TCGTATG30750.027.21677443
CTCGTAT31550.026.81362742
ACGGCTA31750.026.0781932
CGGCTAC32350.025.80316533
CACGGCT32350.025.66202531
AGCACAC33700.025.50520310
GGCTACA34450.023.64254634
TCTCGTA36450.023.27077941
CACGTCT37050.023.01637514
CGTCTGA38000.022.61614416
CATCTCG38000.022.14402839
GAGCACA40050.021.6860319
GCTACAT38800.021.57178735
Produced by FastQC (version 0.11.2)