FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_10.341000000340be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_10.341000000340be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16944523
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27450.027.86996311
CGTATGC28000.027.077244
GTATGCC28300.026.79016145
ACGTCTG32200.024.175215
ACACGTC33550.023.27216313
AGCACAC35850.022.03016710
CTCGTAT34250.022.00616342
TCGTATG34550.021.68343443
CACACGT36250.021.47671712
CACTAGC36300.020.82401831
TAGCTTA37000.020.3708734
TATCTCG38300.020.03160339
ACTAGCT38100.019.9008432
CACGTCT41900.018.74146514
GTCACTA40000.018.67315129
CGTCTGA42150.018.41422316
GAGCACA43900.018.2979959
TCACTAG42400.018.09340930
AGAGCAC44900.018.0901748
CAGTCAC43350.017.69721227
Produced by FastQC (version 0.11.2)