FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l05n01 4tfs_bhtl_1.34100000033ea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l05n01 4tfs_bhtl_1.34100000033ea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14425458
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG6750.013.6747971
GCCGGGG13600.011.7533111
TGGTACC75700.010.93675720
GCGGGGG14650.010.4498991
GCGGGGA16450.010.4013211
GGTACCG48950.010.34109321
CATCGCA59000.010.29627135
TTATATG59250.010.06243723
GCCGGAT20650.010.0301761
TACCGAG50700.010.02873423
GCGGGAT17300.010.0204091
GGTACCT33400.09.901648521
GTACCGA51100.09.81794522
GCGGGTT13300.09.8178671
GCCGGGT14250.09.7952981
GCGGGAG15950.09.7393331
TAACGTA62800.09.6783154
GCGGGGC8150.09.6683241
GCCGAGT17550.09.6211041
GCGGATC41050.09.5966682
Produced by FastQC (version 0.11.2)