FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_input_60.341000000341bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_input_60.341000000341bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13574317
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14650.035.47788611
ACGTCTG15900.032.82868215
CACACGT15800.032.46842212
CACGGCT15600.031.7290831
ACACGTC16550.031.26883113
GTATGCC16600.029.81769245
CGTATGC16600.029.68215844
CGTCTGA17650.029.57371316
TCGGAAG20450.025.4241283
AGCACAC21000.024.85701210
GCTACAT19250.024.77790535
GAGCACA21000.024.7498689
TCTCGTA20800.024.44581641
AGAGCAC21800.023.9448288
CTACATC20000.023.84873236
CTGAACT22200.023.41091719
CATCTCG20450.023.32394239
TCTGAAC22750.023.14162418
ATCTCGT22400.022.09703840
AGTCACG38100.013.93626628
Produced by FastQC (version 0.11.2)