FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_input_5.3410000003417a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_input_5.3410000003417a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14635938
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC205010.14007301752713083TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26100.041.2081911
GTATGCC26550.039.5735945
ACGTCTG26950.039.5710115
CACACGT27050.039.42822612
CGTATGC27050.038.84210244
ACACGTC27500.038.7010813
CGTCTGA28350.037.61688616
TCGGAAG28550.037.2908743
AGCACAC29000.036.93181610
GAGCACA29500.036.3821229
TCTCGTA29650.035.5119341
CGATCAG29750.035.01443533
CTGAACT30850.034.93280419
AGAGCAC31050.034.710888
TCTGAAC30750.034.6805818
ATCTCGT31850.033.4121840
AGATCTC34100.030.74571838
AGTCACG56000.019.40493628
GTCACGA57050.018.77173429
CACGATC59000.018.18944531
Produced by FastQC (version 0.11.2)