FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_input_300.341000000341ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_input_300.341000000341ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17829952
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC230080.1290412896232138TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26400.041.07973511
ACGTCTG26950.039.73798815
CACACGT28100.038.7546312
ACACGTC28150.038.1261813
CGTCTGA28350.037.616916
GTATGCC27900.036.77168345
CGTATGC28000.036.64035444
AGTCACC29150.036.19821528
AGCACAC30300.035.866110
TCGGAAG30250.035.4200253
GAGCACA30850.035.3725369
CTGAACT32100.033.50238419
AGAGCAC32600.033.4737058
TCTCGTA31350.032.7250441
TCTGAAC33950.031.61050818
ATCTCGT32550.031.51858340
AATCTCG32800.031.00397939
TGTAATC33450.030.0652136
CTTGTAA41550.024.63730634
TCCCTAC6300.016.0713219
Produced by FastQC (version 0.11.2)