FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_input_30.341000000341a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_input_30.341000000341a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13716255
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC149730.10916244995445186TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19850.038.19904711
ACGTCTG21800.035.39923515
CACACGT21900.034.6233412
GTATGCC22550.033.7227745
ACACGTC22800.033.6512413
CGTATGC23050.033.08886344
CGTCTGA24000.032.2481716
CACTAGC24050.030.87108631
GAGCACA25750.029.7959429
AGCACAC25850.029.68067710
TCGGAAG25850.029.1706663
GTCACTA26000.028.90188629
AGAGCAC26950.028.3857338
AGTCACT27700.027.69667828
TCTCGTA28150.027.17441
TCTGAAC28700.027.12369718
ATCTCGT28650.026.54270440
ACTAGCT28000.026.51605832
GCTTATC28800.026.0919836
CTGAACT29800.026.04698619
Produced by FastQC (version 0.11.2)