FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_input_1.3410000003411d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_input_1.3410000003411d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14110283
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16250.034.75435311
ACGTCTG16650.033.78215
CACACGT17950.031.58820312
CGTATGC17950.031.20983144
ACACGTC18700.030.44162213
GTATGCC18300.030.2440945
GTCACAC18650.029.55587229
CGTCTGA19500.028.9600116
TCGGAAG21750.025.4573063
GAGCACA22500.025.3001889
AGCACAC24500.023.32670410
AGAGCAC24550.023.2791968
TCTGAAC24550.022.81940818
AATCTCG24750.022.54410639
TCTCGTA25450.022.10083841
CTGAACT26350.021.85827419
TCACACT25350.021.65550830
CACACTT26050.021.41906431
ATCTCGT26900.020.90952940
TCGTATG46800.012.78769543
Produced by FastQC (version 0.11.2)