FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_input_0.341000000340cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_input_0.341000000340cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12178645
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11700.038.6543911
ACACGTC12800.035.50831213
CACACGT12750.035.4710912
ACGTCTG12950.035.26844815
GTATGCC12850.033.96697645
CGTCTGA13400.033.9161616
CGTATGC13200.033.4072344
AGTCACC12600.033.39099528
AGCACAC14700.031.224810
CATCTCG14150.030.68733639
TCGGAAG14850.030.464733
GAGCACA15150.030.0002989
AGAGCAC15550.029.3732838
TCTCGTA15250.028.47382241
CTGAACT16150.028.00153419
CCAGATC15650.026.88348633
CACCAGA15650.026.59596331
TCTGAAC17200.026.55375318
ATCTCGT16700.026.40571440
ACCAGAT16650.024.99860632