FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_chip_60.3410000003406e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_chip_60.3410000003406e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9714871
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2471862.5444084640959206TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG274850.043.46719715
GCACACG274300.043.41625211
GTATGCC271500.043.3403545
CGTCTGA276600.043.09457816
ACACGTC276650.043.04745513
CGTATGC273500.043.01519844
CACACGT277150.042.97791312
CACAGTG274450.042.7679333
GTCACAC274700.042.72900829
AGCACAC279450.042.535410
GAGCACA279900.042.475059
TCGGAAG279550.042.422573
TCTCGTA277000.042.41482541
AGAGCAC280800.042.3549428
CTGAACT280900.042.19438619
CAGTGAT279100.041.95059235
TCTGAAC283300.041.94017418
ACAGTGA278600.041.8724434
ACACAGT280100.041.69639632
ATCTCGT282350.041.68286540