FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_chip_5.34100000034001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_chip_5.34100000034001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10246223
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2892672.823157372233651TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC328450.043.84655845
CGTATGC328950.043.72519344
CGTCTGA333250.043.6749516
ACACGTC332700.043.6694113
ACGTCTG333700.043.62279515
CACACGT334000.043.56028412
TCTCGTA331300.043.41504341
GCACACG335750.043.3801511
TCGGAAG335950.043.0106773
CACTTAG332900.042.94280231
ATCTCGT334950.042.93522340
CATCTCG335500.042.65024639
TAGGCAT334300.042.6350935
AGTCACT336150.042.5142328
CTCGTAT339550.042.4330842
AGCACAC343050.042.3578210
TCGTATG340850.042.3438543
TCTGAAC342250.042.34155318
AGAGCAC343950.042.2535258
GCATCTC339450.042.12743438