FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_chip_300.341000000340a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_chip_300.341000000340a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12471480
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC8849407.095709570957095TruSeq Adapter, Index 6 (100% over 51bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG143440.11501441689358441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1006700.044.3182311
ACGTCTG1004200.044.2871615
ACACGTC1005850.044.22370513
CACACGT1007650.044.22285512
CGTCTGA1005650.044.1696116
GAGCACA1008800.044.102779
AGCACAC1009150.044.0852510
AGAGCAC1009300.044.0363438
TCGGAAG1005600.044.0352023
GTATGCC990200.044.0107545
TCTGAAC1007250.043.91129318
CTGAACT1007000.043.9043219
CACGCCA985700.043.88527731
CGTATGC993250.043.85748744
TCACGCC996250.043.84510430
GCCAATA980100.043.69987534
TCTCGTA996650.043.6536941
ATCTCGT1000700.043.51748340
AGTCACG1003750.043.5085328
CTCCAGT1014600.043.3226624
Produced by FastQC (version 0.11.2)