FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_chip_1.34100000033fdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_chip_1.34100000033fdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11867658
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC6567795.5341921716989155TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG746550.043.92403811
ACGTCTG746750.043.8616615
CGTCTGA748300.043.71969216
ACACGTC748400.043.70121413
GTATGCC736600.043.61692445
CACACGT751250.043.6162912
CGTATGC737400.043.52689444
AGCACAC752100.043.52511610
GAGCACA753300.043.4946069
AGTCACC738500.043.42854728
AGAGCAC755850.043.407418
ACCGATG739600.043.36091232
TCGGAAG754700.043.3571663
CTGAACT753100.043.306619
TCTCGTA741950.043.27512441
GTCACCG748200.043.20531529
TCTGAAC755000.043.1916618
ATCTCGT746450.042.9991740
CCGATGT748350.042.9892133
CGATGTA744050.042.97458334