FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l04n01 tga1_chip_0.34100000033fa6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l04n01 tga1_chip_0.34100000033fa6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9936777
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC628790.6327906925957985TruSeq Adapter, Index 1 (100% over 51bp)
ACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG116470.1172110433795586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC74950.040.7945444
GTATGCC75050.040.7102145
ACGTCTG77850.040.11340315
GCACACG78300.039.827811
ACACGTC78950.039.44289413
CGTCTGA79200.039.37283316
CACACGT79850.039.167412
TCTCGTA79000.038.7031841
AGCACAC81950.038.05370710
TCTGAAC82600.037.69749518
GTCACAT81100.037.6177729
GAGCACA83250.037.5946049
CTGAACT83350.037.4122719
ATCTCGT82850.036.82319340
ATCACGA83200.036.5330834
AGAGCAC86600.036.1922768
TCGGAAG88300.035.2545053
CTCGTAT91200.033.72313742
TCGTATG92000.033.60107443
TCACGAT91800.033.60075435
Produced by FastQC (version 0.11.2)