FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8441b-062415.3410000000d893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8441b-062415.3410000000d893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15286457
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3383912.2136653378869937No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2802301.8331913012936876No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1181400.7728409532699434TruSeq Adapter, Index 6 (100% over 51bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC635570.41577325602655996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA193450.046.5461921
TTCCAGG420400.046.2771033
TATTCGT358750.046.0465051
CTTCCAG427400.045.6339262
ACTTCCA443750.044.312071
TCGGAAG201950.044.1989023
ATTCGTT376150.043.9049532
TTCGTTG374350.043.888523
ATCGGAA205000.043.793142
CCTCGGG383950.042.70310615
CCAGGGA455050.042.5481645
CTCGGGG386050.042.5460316
TAAGCCG459650.042.43684416
TCGGGGA469650.042.3972517
TCCAGGG456800.042.3851664
CTGACCC458300.042.33458740
CCTGACC458950.042.22439639
GGCGGTC466000.042.13100438
CAGGGAT461950.041.954736
AGCCGAT463700.041.90780318
Produced by FastQC (version 0.11.2)