FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8441a-062415.3410000000d850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8441a-062415.3410000000d850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41218941
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG834420.20243605967460443TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA272900.042.9522741
CGTCCCG224250.041.09136235
GCACACG288750.039.38882411
GTCCCGA189350.039.3159436
CCCGTCC254400.038.9208933
ACCCGTC258150.038.82198732
TCACCCG268550.038.3646330
CCGTCCC258550.037.73552334
CACACGT303450.037.5468412
ACACGTC303250.037.4686713
CACCCGT271750.037.21548531
TCGGAAG312500.037.1749233
ACGTCTG304200.037.14539715
CACGTCT308300.036.81198514
CCCGATC184600.036.6921938
CGTCTGA310300.036.50148816
AGCACAC318500.035.94727310
GTCACCC293100.035.78258529
ATCGGAA329250.035.526622
GAACTCC317050.035.3158921
Produced by FastQC (version 0.11.2)