FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8440d-062415.3410000000d645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8440d-062415.3410000000d645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23282777
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC16390157.039602707185659TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA7919223.4013210709358255No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC6173402.6514878358367646No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1610500.691713020315403No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGCC265750.11414016463757738TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2269200.051.0005461
TCGGAAG2246800.050.8110473
ATCGGAA2269250.050.6255872
TTCCAGG938700.048.345173
TATTCGT756900.048.014531
CTTCCAG949200.047.9653172
ACTTCCA976100.047.0899121
ATTCGTT781450.046.395482
TTCGTTG782450.046.108663
GCACACG2507100.044.109111
ACACGTC2507900.043.9874513
AGCACAC2511950.043.97385410
CACACGT2513750.043.9507212
GAGCACA2513950.043.877679
CACGTCT2521350.043.84053814
ACGTCTG2504250.043.79739415
AGAGCAC2520000.043.748448
CGGAAGA2538600.043.7285734
CGTCTGA2494700.043.6989116
GTATGCC2394700.043.5565345
Produced by FastQC (version 0.11.2)