FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8440c-062415.3410000000d5f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8440c-062415.3410000000d5f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31260407
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC4754321.5208759118203419TruSeq Adapter, Index 12 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA481670.15408308663415674No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC447570.14317471938225243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA686850.049.4451751
TCGGAAG707350.047.474393
ATCGGAA730050.046.2636452
GCACACG759300.043.16183511
ACACGTC766350.042.5407613
CACACGT769450.042.52873212
ACGTCTG766850.042.36989215
CACGTCT773850.042.2616714
AGCACAC776150.042.17002510
CGTCTGA769700.042.12038816
GAGCACA781350.041.8151939
GTATGCC728600.041.53237545
AGAGCAC788500.041.4049158
TCCAGTC771500.041.3480225
CAGTCAC760450.041.33346627
GAACTCC783000.041.1425221
CCAGTCA764200.041.01686526
AGTCACC747350.040.9689728
CGTATGC739500.040.9168644
GTCTGAA795150.040.67413317
Produced by FastQC (version 0.11.2)