FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8440a-062415.3410000000d596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8440a-062415.3410000000d596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15908609
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG5006603.14710104447221TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA788950.050.745441
TCGGAAG792200.049.793423
ATCGGAA805450.049.310922
GCACACG875400.043.63109611
CACACGT883150.043.24821512
ACGTCTG877350.043.2351715
CACGTCT883200.043.22332414
ACACGTC881600.043.21803713
AGCACAC883750.043.1932210
GAGCACA884500.043.131399
CGTCTGA877750.043.088616
AGAGCAC888200.042.8764088
GAACTCC874500.042.61580721
TCCAGTC876550.042.4955225
CAGTCAC865050.042.45480727
CCAGTCA862550.042.3917626
ACTCCAG879200.042.3344723
TCGTATG761500.042.31154345
GTCTGAA892250.042.26592617
CGGAAGA908850.042.2279554