FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8383b-062415.3410000000d8e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8383b-062415.3410000000d8e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069528
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA94870.3090703196061414No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC77760.25332885055943455No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC36500.11891079019315022TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGA14950.037.42884417
CTCGGGG11900.036.3436616
TAAGCCG16300.036.106916
CCTCGGG12050.035.7062415
CTGACCC16050.035.2797540
GGCGGTC15650.035.1826238
CCTGACC16400.035.06884439
CATCCCT16900.034.16320835
CGGTCTC16350.033.81432340
GCGGTCT16550.033.53875439
GCACACG8700.033.05608411
GTCTCCC16600.033.03809742
AGGCGGT16900.032.9760737
AGCCGAT17750.032.90452618
TGACCCT17350.032.76586541
CCAGGGA17500.032.7435
CCCTGAC17800.032.3106238
ATCCCTG18100.032.14456636
CAGGCGG17250.032.0485336
CACACGT9100.031.84805512
Produced by FastQC (version 0.11.2)