FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8115b-062415.3410000000d737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8115b-062415.3410000000d737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5843203
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA368790.6311435697168146No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC305820.5233773326033684No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG168160.28778736593611415TruSeq Adapter, Index 18 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC78260.13393339235347462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGA54900.041.66621417
GGCGGTC54300.041.46895638
CTCGGGG45150.041.42983616
CCTCGGG45200.041.33432815
CAGGCGG56450.039.9290336
GTCTCCC56700.039.91230842
GATCGGA34100.039.827171
CCAGGCG56250.039.79355635
CCCAGGC56650.039.6734
ACGTCCG29050.039.60090632
AGGCGGT57050.039.4309437
CTGACCC55350.039.39468840
TCCTCGG48000.039.15740614
CCTGACC55700.039.10577839
ATAAGCC57250.038.55360815
GCACACG33000.038.51165811
AGTCACG31500.038.43181228
TGACCCT56750.038.38387741
CCAGGGA56900.038.3656545
CGTCCGC30150.038.3039933
Produced by FastQC (version 0.11.2)