FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8115a-062415.3410000000d6be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8115a-062415.3410000000d6be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24676992
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62000.030.34917411
GATCGGA65650.029.6507171
ACACGTC66650.028.2652413
CACACGT66500.028.19489512
ACGTCTG69600.026.93821515
CACGTCT71800.026.48653414
CGTCTGA74400.025.32012416
AGCACAC76250.024.61833610
ACTGACC69200.023.64591832
GAGCACA80600.023.3449979
CAGTCAC74950.023.31918127
TCGGAAG87150.022.629983
AGAGCAC85900.022.3977368
GAACTCC82850.022.36033221
TCCAGTC84600.021.58158125
GTATGCC75850.021.34010345
CCAGTCA84400.021.15732226
CGTATGC78800.020.51274544
ATCGGAA97450.020.3441942
GTCTGAA96500.020.02967317