FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8104b-062415.3410000000d81c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8104b-062415.3410000000d81c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758399
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1318082.770007307079545No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1067762.243948016969573No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG976112.0513412179180435TruSeq Adapter, Index 19 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC259910.5462131275666459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA155200.049.3020741
TCGGAAG153350.049.2183
ATCGGAA156400.048.5555652
TTCCAGG160850.047.935133
TATTCGT133200.047.085161
CTTCCAG165300.046.8478972
ATTCGTT137350.045.6580472
ACTTCCA172150.045.288381
TTCGTTG138150.045.1317943
GCACACG169650.043.4978411
CACACGT171200.043.1691312
ACACGTC169600.043.16888413
AGCACAC171650.043.02998410
CCAGGGA178350.043.007365
CACGTCT170500.042.95409414
ACGTCTG169500.042.85102515
CTCGGGG146800.042.84073316
GAGCACA172100.042.826799
TCCAGGG179250.042.8038644
CGTCTGA169800.042.78844516
Produced by FastQC (version 0.11.2)