FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l03n01 ah8104a-062415.3410000000d7bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l03n01 ah8104a-062415.3410000000d7bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25155020
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1970630.7833943284481587TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA298150.047.272011
TCGGAAG315150.044.1592563
ATCGGAA329900.042.513892
GCACACG327400.041.64028511
ACACGTC332950.040.85243213
CACACGT334000.040.83080712
ACGTCTG332350.040.7305215
CACGTCT338050.040.36841614
CGTCTGA337900.039.91636716
AGCACAC342650.039.77990310
GAGCACA345650.039.3636939
TATCTCG317350.039.24070439
TCCAGTC338350.038.92020425
GTATGCC322300.038.86937745
CAGTCAC334650.038.84446327
GAACTCC343000.038.8085421
AGAGCAC350500.038.7363178
CCGATGT320200.038.68960633
CCAGTCA338150.038.29736726
CACCGAT323000.038.17468631
Produced by FastQC (version 0.11.2)