FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8440b-062415.3410000000e27c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8440b-062415.3410000000e27c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12074284
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3251792.6931534822271863No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2666382.2083131388991677TruSeq Adapter, Index 2 (100% over 51bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2591902.1466283218118773No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC610750.5058270950062133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG322150.043.5639511
ACACGTC324300.043.1849513
CACACGT324800.043.15310712
TCGGGGA354250.042.97235517
ACGTCTG325950.042.96812415
CTCGGGG286900.042.88884716
CACGTCT326750.042.87687314
AGCACAC327300.042.8716110
CCAGGGA360350.042.742155
CCTCGGG288200.042.61643615
GGCGGTC355850.042.5975438
TAAGCCG361550.042.57765216
CGTCTGA329150.042.49658216
TCCAGGG361700.042.476834
CTGACCC360350.042.4740940
GATCGGA332700.042.465871
CCTGACC360350.042.41578339
CCGATGT316800.042.4054533
GTCTCCC358800.042.3502842
GAGCACA331950.042.3320549