FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8383a-062415.3410000000e2a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8383a-062415.3410000000e2a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216668
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1903915.91888873828446TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA215750.044.1902661
GCACACG214050.044.15329411
CACACGT214450.044.06044412
ACACGTC215600.043.90890513
ACGTCTG216250.043.8407115
CGTCTGA216100.043.82453516
GAGCACA215900.043.7749529
CACGTCT216400.043.75901814
AGCACAC215900.043.7436910
TCGGAAG218350.043.648333
AGAGCAC217750.043.527028
GTATGCC211950.043.5189345
GAACTCC216450.043.40860421
TCCAGTC215450.043.35473325
ATCGGAA220000.043.341432
CAGTCAC215500.043.3167227
CCAGTCA215100.043.30313526
GTCTGAA218850.043.2841317
AGTCACC214250.043.12839528
GTCACCT214000.043.08415629
Produced by FastQC (version 0.11.2)